安徽地方猪TLR4基因第3外显子的SNP分析

doi:10.11843/j.issn.0366-6964.2014.11.005

Abstract

Abstract:

In this study，using the PCR-SSCP method，the genetic variations in exon 3 of TLR4 were detected among 5 pig populations including Wannan Black，Wei，Anqing Liu White，Huoshou Black and Landrace pigs，the total individual number was 354.This study was conducted to analyze the polymorphisms of exon 3 of TLR4 in Anhui native pig breeds and aimed to provide a theoretical foundation for further research on the role of TLR4 in immune and defense system.Two SNPs were found，including G417A and C1027A.G417A was a synonymous SNP site，2 genotypes (GG，GA) were detected only in Wannan Black，Wei and Anqing Liu White pigs，the 3 populations were all in low polymorphism situation，and in Hardy-Weinberg equilibrium (P>0.05).C1027A was a nonsynonymous SNP site and changed the character of amino acid，3 genotypes (CC,CA，AA) were detected in Wannan Black，Wei，Anqing Liu White，Huoshou Black and Landrace pigs，the 5 populations were all in average polymorphism situation，and in Hardy-Weinberg equilibrium (P>0.05).The χ² test results of independence for the genotypes distribution of each SNP locus showed that the genotype frequencies for Landrace and Wannan Black，Wei，Anqing Liu White，Huoshou Black pigs were significantly different (P<0.01) at G417A and C1027A loci.These results indicated that the exon 3 sequences of TLR4 were relatively conservative in Anhui native pig breeds，and these populations had low polymorphism and genetic variation.In addition，the association between the differential genotypes distribution of C1027A locus in multiple pigs and the different disease resistance capability for pig breeds was worthy of further research.

CLC Number:

S828
S813.3

DING Yue-yun，ZHU Wei-hua，XUE Wei-wei，MENG Yun，HUANG Long，ZHANG Xiao-dong，YIN Zong-jun. Single Nucleotide Polymorphism (SNP) Analysis of TLR4 Gene Exon 3 in Anhui Native Pig Breeds[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1767-1774.

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References

［1］KONO D H，BACCALA R，THEOFILOPOULOS A N.TLRs and interferons：a central paradigm in autoimmunity ［J］.Curr Opin Immunol，2013，25(6):720-727.
［2］SANG Y，YANG J，ROSS C R，et al.Molecular identification and functional expression of porcine Toll-like receptor (TLR) 3 and TLR7 ［J］.Vet Immunol Immunopathol，2008,125(1-2):162-167.
［3］NAIR A R，MASSON G S，EBENEZER P J，et al.Role of TLR4 in lipopolysaccharide-induced acute kidney injury：protection by blueberry ［J］.Free Radic Biol Med,2014，doi：10.1016/j.freeradbiomed.2014.03.012.
［4］CALIL I L，ZARPELON A C，GUERRERO A T,et al.Lipopolysaccharide induces inflammatory hyperalgesia triggering a TLR4/MyD88-dependent cytokine cascade in the mice paw［J］.PLoS ONE，2014,9(3):e90013.
［5］STAMM LV，DRAPP R L.TLR2 and TLR4 mediate the TNFα response to Vibrio vulnificus biotype 1［J］.Pathog Dis，2014，doi：10.1111/2049-632X.12154.
［6］WANG X P，LUORENG Z M，GAO S X，et al.Haplotype analysis of TLR4 gene and its effects on milk somatic cell score in Chinese commercial cattle ［J］.Mol Biol Rep，2014，doi:10.1007/s11033-014-3088-7.
［7］SHINKAI H，OKUMURA N，SUZUKI R,et al.Toll-like receptor 4 polymorphism impairing lipopolysaccharide signaling in Sus scrofa，and its restricted distribution among Japanese wild boar populations ［J］.DNA Cell Biol，2012，31(4):575-581.
［8］THOMAS A V，BROERS A D,VANDEGAART H F,et al.Genomic structure，promoter analysis and expression of the porcine (Sus scrofa) TLR4 gene［J］.Mol Immunol，2006，43(6):653-659.
［9］PALERMO S，CAPRA E，TORREMORELL M，et al.Toll-like receptor 4 genetic diversity among pig populations［J］.Anim Genet，2009，40(3)：289-299.
［10］SHINKAI H，TANAKA M，MOROZUMI T，et al.Biased distribution of single nucleotide polymorphisms (SNPs) in porcine Toll-like receptor 1(TLR1)，TLR2，TLR4，TLR5，and TLR6 genes［J］. Immuno Genet，2006，58(4)：324-330.
［11］周波，刘传武，虞德兵，等.用PCR-SSCP 方法检测猪 Toll 样受体 4 (TLR4)基因外显子3 的 SNP［J］.畜牧与兽医，2008，40(6)：26-30.
［12］翟春媛，杨秀芹，李海涛，等.猪 TLR4 基因的变异位点分析［J］.中国畜牧杂志，2011,47(3):18-21.
［13］潘章源,叶兰,朱璟,等.猪TLR4 基因外显子 1 新等位基因的分离及遗传变异分析［J］.遗传,2011,33(2):163-167.
［14］邱小田，李玉华，李何君，等.猪Toll like Receptor4(TLR4)的定位和组织表达［J］.农业生物技术学报，2007，15(1)：37-40.
［15］包文斌，潘章源，朱璟，等.猪TLR4基因在F18大肠杆菌抗性型和敏感型资源群体间的差异表达分析［J］.畜牧兽医学报，2011，42(2)：278-283.
［16］YANG X Q，MURANI E，PONSUKSILI S,et al.Association of TLR4 polymorphism with cytokine expression level and pulmonary lesion score in pigs ［J］.Mol Biol Rep，2012，39(6):7003-7009.
［17］刘筱，方晓敏，邹晓龙，等.苏钟猪TLR4多态性及编码区C1027A功能分析［J］.中国农业科学,2012,45(6):1206-1214.
［18］杨秀芹，陈月婵，汪亮,等.猪 Toll 样受体 4 基因 SNPs 功能分析［J］.遗传，2012,34(8):1050-1056.
［19］刘筱，方晓敏，任守文，等.猪 Toll 样受体4 基因(TLR4)外显子 SNP 检测及生物信息学分析［J］.江苏农业学报,2011,27(4):782-789.
［20］GIORGIO T，ALAN S.Cooperation of Toll-like receptor signals in innate immune defense ［J］.Nat Rev Immunol，2007，7(3)：179-190.
［21］UENISHI H，SHINKAI H.Porcine Toll-like receptors：The front line of pathogen monitoring and possible implications for disease resistance ［J］. Dev Comp Immuno，2009，33：353-361.
［22］ALVAREZ B，REVILLA C，CHAMORRO S，et al.Molecular cloning，characterization and tissue expression of porcine Toll-like receptor 4［J］.Dev Comp Immunol，2006，30：345-355.
［23］PARK B S，SONG D H，KIM H M，et al.The structural basis of lipopolysaccharide recognition by the TLR4-MD-2 complex［J］.Nature，2009，458(7242)：1191-1195.
［24］KIM H M，PARK B S，KIM J I，et al.Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist Eritoran［J］.Cell，2007，130(5)：906-917.

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Single Nucleotide Polymorphism (SNP) Analysis of TLR4 Gene Exon 3 in Anhui Native Pig Breeds

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